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KMID : 0370219980420010053
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Yakhak Hoeji
1998 Volume.42 No. 1 p.53 ~ p.58
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Immobilization of Arylsulfate Sulfotransferase Obtained from Haemophilus K-12
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이남수/Lee NS
김병택/최승기/김동현/Kim BT/Choi SK/Kim DH
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Abstract
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A novel type of sulfotransferase, arylsulfate sulfotransferase (EC 2.8.2.22) purified from Haemophilus K-12, an intestinal bacterium of a mouse, was immobilized onto AH-S epharose 4B, CH-Sepharose 4B and DEAE-celluose. The enzyme was stabilized for storage more markedly by covalent immobilization onto AH-Spharose 4B or CH-Sepharose 4B and by adsorptive immobilization onto DEAE-cellulose than the free enzyme. The optimal pH and acceptor substrate specificity of immobuized enzyme were similar to those of the free enzyme.
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